Cortical columns interact through dynamic routing of neuronal activity. To monitor these interactions, we developed the Multiplane Mesoscope which combines three established microscopy technologies: time-division multiplexing, remote focusing, and random-access mesoscopy. The Multiplane Mesoscope allowed us to study cortical column interactions in excitatory and inhibitory subpopulations in behaving mice. We found that distinct cortical subnetworks represent expected and unexpected events, suggesting that expectation violations modify signal routing across cortical columns, and establishing the Multiplane Mesoscope as a unique platform to study signal routing.
bioRxiv Subject Collection: Neuroscience