February 25, 2021

Molecular and genetic approaches for assaying human cell type synaptic connectivity

Prospective and post-hoc molecular identification of specific neuron types is essential for functional studies of cellular and synaptic properties. We demonstrate a thick brain slice mFISH technique applied to multi-patch-clamp recordings in human cortical slices obtained from neurosurgical-excised tissue to reveal the molecular and morpho-electric properties of synaptically connected neurons, both with and without prospective AAV based genetic labeling. This quadruple modality methodology should be extensible to other local brain circuits in many organisms.

 bioRxiv Subject Collection: Neuroscience

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