May 9, 2021

Contingent-behavior assay shows zebrafish preference for alcohol is biphasic

<p>Alcohol use disorders are poorly understood, complex, multifactorial phenomena with a very large footprint within the global burden of diseases. Methods to study the development of addiction have primarily focused on contingent behaviors in rodents. Here, we report the development of an accessible, two-choice self-administration zebrafish assay (SAZA). Using this assay, we demonstrated that, while zebrafish avoid higher concentrations of alcohol, they are attracted to low concentrations. Pre-exposure to alcohol did not change this relative preference, and acute exposure to a deterrent decreased self-exposure to preferred concentration. A pigment mutant used in whole-brain imaging studies also displayed a similar relative preference profile but administrated substantially larger volumes of alcohol. The presence of a biphasic response (hormesis) in zebrafish validated a key aspect of vertebrate responses to alcohol. Our findings reveal the underappreciated usefulness of zebrafish in studying the neurogenetics of alcohol addiction and the discovery of novel alcohol deterrents.</p>
<p> bioRxiv Subject Collection: Neuroscience</p>
<p> <a href="http://biorxiv.org/cgi/content/short/2021.05.04.442404v1?rss=1">Read More</a></p>

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